Researchers have published various protocol documents on the website protocols.io to help those new to working with anaerobic fungi:

Unifying/Standardizing Basic Methodologies for the Isolation and Characterization of Anaerobic Fungi (Neocallimastigomycota) (2026)

High‑Molecular‑Weight DNA Extraction from Anaerobic Gut Fungi for PacBio Long‑Read Genome Sequencing (2026)

Defined medium for Neocallimastigomycota (2024)

Medium without Clarified Rumen Fluid for Neocallimastigomycota (2023)

Methods for Genomic Characterization and Maintenance of Anaerobic Fungi (2018)